Other EE ELISA Kit orb781892
https://www.biorbyt.com/other-ee-elisa-kit-orb781892.html
Catalog Number | orb781892 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ethinylestradiol(EE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ethinylestradiol(EE). |
Tested applications | ELISA |
Reactivity | All |
Range | 31.25-2000 pg/mL |
Concentration | 2000 pg/mL |
Sensitivity | 9.34 pg/mL |
Protein Length | 2h |
Alternative names | Ethinyl Estradiol ELISA Kit, Ethynyl Estradiol ELI Read more... |
Note | For research use only |
Application notes | Standard: 2000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with EE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EE in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |