Human MBL ELISA Kit orb781894
https://www.biorbyt.com/human-mbl-elisa-kit-orb781894.html
| Catalog Number | orb781894 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Mannose Binding Lectin(MBL) protein. |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 6.25-400 ng/mL |
| Concentration | 400 ng/mL |
| Sensitivity | 2.45 ng/mL |
| Protein Length | 3.5h |
| Alternative names | MBL2 ELISA Kit, COLEC1 ELISA Kit, HSMBPC ELISA Kit Read more... |
| Note | For research use only |
| Application notes | Standard: 400 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |