Other 5-Me-THF ELISA Kit orb781896
https://www.biorbyt.com/other-5-me-thf-elisa-kit-orb781896.html
| Catalog Number | orb781896 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Methyltetrahydrofolate(5-Me-THF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Methyltetrahydrofolate(5-Me-THF). |
| Tested applications | ELISA |
| Reactivity | All |
| Range | 1.41-90 ng/mL |
| Concentration | 90 ng/mL |
| Sensitivity | 0.43 ng/mL |
| Protein Length | 2h |
| Alternative names | 5-Me-THFA ELISA Kit, 5-Me-H4FA ELISA Kit, 5-Me-THF Read more... |
| Note | For research use only |
| Application notes | Standard: 90 ng/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Me-THF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Me-THF in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |