Other PAF ELISA Kit orb781909
https://www.biorbyt.com/other-paf-elisa-kit-orb781909.html
Catalog Number | orb781909 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Platelet Activating Factor(PAF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Platelet Activating Factor(PAF). |
Tested applications | ELISA |
Reactivity | All |
Range | 46.88-3000 pg/mL |
Concentration | 3000 pg/mL |
Sensitivity | 14.4 pg/mL |
Protein Length | 2h |
Alternative names | PAF-Acether ELISA Kit, AGEPC ELISA Kit, Acetyl-Gly Read more... |
Note | For research use only |
Application notes | Standard: 3000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PAF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAF in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |