Animal APOA4 ELISA Kit orb781929
https://www.biorbyt.com/animal-apoa4-elisa-kit-orb781929.html
| Catalog Number | orb781929 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A4(APOA4). |
| Tested applications | ELISA |
| Reactivity | Bovine |
| Range | 1.57-100 ng/mL |
| Concentration | 100 ng/mL |
| UniProt ID | Q32PJ2 |
| Sensitivity | 0.69 ng/mL |
| Protein Length | 3.5h |
| Alternative names | ApoA-IV ELISA Kit, ApoAIV ELISA Kit, Apo-A4 ELISA Read more... |
| Note | For research use only |
| Application notes | Standard: 100 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle APOA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle APOA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle APOA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle APOA4 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |