Other PGI2 ELISA Kit orb781943
https://www.biorbyt.com/other-pgi2-elisa-kit-orb781943.html
Catalog Number | orb781943 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostacyclin(PGI2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostacyclin(PGI2). |
Tested applications | ELISA |
Reactivity | All |
Range | 3.13-200 pg/mL |
Concentration | 1000 pg/mL |
Sensitivity | 0.93 pg/mL |
Protein Length | 2h |
Alternative names | prostaglandin I2 ELISA Kit Read more... |
Note | For research use only |
Application notes | Standard: 200 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGI2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGI2 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |