Other SAM ELISA Kit orb781945
https://www.biorbyt.com/other-sam-elisa-kit-orb781945.html
Catalog Number | orb781945 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with S-Adenosyl Methionine(SAM) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S-Adenosyl Methionine(SAM). |
Tested applications | ELISA |
Reactivity | All |
Range | 15.63-1000 ng/mL |
Concentration | 1000 ng/mL |
Sensitivity | 4.84 ng/mL |
Protein Length | 2h |
Alternative names | SAMe ELISA Kit, SAM-e ELISA Kit, S-Adenosylmethion Read more... |
Note | For research use only |
Application notes | Standard: 1000 ng/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SAM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SAM in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |