Other DAG ELISA Kit orb781983
https://www.biorbyt.com/other-dag-elisa-kit-orb781983.html
Catalog Number | orb781983 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Diacylglycerol(DAG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diacylglycerol(DAG). |
Tested applications | ELISA |
Reactivity | All |
Range | 1562.5-100000 pg/mL |
Concentration | 100000 pg/mL |
Sensitivity | 141 pg/mL |
Protein Length | 2h |
Alternative names | DG ELISA Kit, Diglyceride ELISA Kit Read more... |
Note | For research use only |
Application notes | Standard: 100000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DAG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DAG in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |