Mouse sCD14(SolubleCluster of Differentiation 14) ELISA Kit orb1146713
https://www.biorbyt.com/mouse-scd14solublecluster-of-orb1146713.html
| Catalog Number | orb1146713 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SolubleCluster of Differentiation 14(sCD14). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SolubleCluster of Differentiation 14(sCD14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SolubleCluster of Differentiation 14(sCD14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SolubleCluster of Differentiation 14(sCD14) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Mouse |
| Range | 1.56-100 ng/mL |
| Concentration | 100 ng/mL |
| Sensitivity | 0.94 ng/mL |
| Protein Length | 3.5h |
| Alternative names | CD14 Antigen, Monocyte Differentiation Antigen CD14 Read less |
| Note | For research use only |
| Application notes | Standard: 100 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sCD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sCD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sCD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse sCD14 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
