Mouse RSPO2 ELISA Kit orb781989
https://www.biorbyt.com/mouse-rspo2-elisa-kit-orb781989.html
| Catalog Number | orb781989 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 2(RSPO2). |
| Tested applications | ELISA |
| Reactivity | Mouse |
| Range | 78.13-5000 pg/mL |
| Concentration | 5000 pg/mL |
| UniProt ID | Q8BFU0 |
| Sensitivity | 29 pg/mL |
| Protein Length | 3.5h |
| Alternative names | Roof plate-specific spondin-2 ELISA Kit Read more... |
| Note | For research use only |
| Application notes | Standard: 5000 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RSPO2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RSPO2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RSPO2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RSPO2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Expiration Date | Please enquire. |