Human N-MID-OT(N-MIDOsteocalcin) ELISA Kit orb1146745
https://www.biorbyt.com/human-n-mid-otn-midosteocalci-orb1146745.html
| Catalog Number | orb1146745 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-MIDOsteocalcin(N-MID-OT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-MIDOsteocalcin(N-MID-OT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-MIDOsteocalcin(N-MID-OT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-MIDOsteocalcin(N-MID-OT) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 0.63-40 ng/mL |
| Concentration | 40 ng/mL |
| Sensitivity | 0.39 ng/mL |
| Protein Length | 3.5h |
| Alternative names | N-MID-OT Read less |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |