主要用于人的原代细胞培养（包括皮肤、口腔、眼角膜、乳腺细胞等）,对应以前的货号CnT-07,CnT-20,CnT-24等 详情见http://cellntec.com/products/cnt-pr/#datasheet
CNT-PR
CnT-Prime，上皮细胞培养基（增殖）
CnT-Prime是一种完全定义的低钙（0.07 mM）中等配方，旨在使原发性上皮细胞（皮肤，角膜，口腔，乳腺）的最佳分离，生长和增殖。 它完全没有动物或人源性成分。 CnT-Prime包含全系列的氨基酸，矿物质，维生素和有机化合物，加上生长因子以提供强烈的细胞增殖，以及辅助因子来改善生长因子与细胞表面受体的结合。CnT-Prime还含有PCT因子，可以使增殖祖细胞的保留最大化，延长寿命，并通过分化减少损失。 它不含酚红或抗生素/抗霉素。
种类
开发用于原代人类细胞（皮肤，口腔，角膜，乳腺）。 也可以用于其他物种，包括老鼠。
组织类型皮肤，口腔，角膜，乳腺，膀胱
包装尺寸：
500毫升/瓶，充分补充生长因子。 不需要进一步添加。
产品用途
专门用于在完全限定的环境中提供高效分离，强烈增殖和延长原代上皮细胞的寿命。 包括PCT因子增加了增殖祖细胞的保留，并且通过分化使损失最小化。 诱导分化时不推荐使用。 对于差异化工作，请使用CnT-PR-D培养基。
培养条件
该介质设计用于5％二氧化碳气氛。 有关建议的隔离，传播，分化和冻结方案，请访问的资源部分。
注意
对于常规细胞培养，CELLnTEC不建议使用抗生素/抗霉素。 隔离期间，推荐使用抗生素/抗菌药，直到P1结束。
存储
储存冷冻至-15℃以下。 为了最好的日期，请参阅标签。 为了制备使用的介质，优选在4℃下解冻过夜。 或者，培养基可在室温下解冻约5-6小时。 如果在室温下解冻，确保解冻过程结束时内容物不会超过15oC。 不要在热水浴中解冻。 一旦解冻，培养基在黑暗中储存于4oC时，保质期为6周。 某些培养基组分对光非常敏感。 始终尽量减少曝光。
质量控制
通过渗透压，pH和各种离子的浓度测试培养基组成。 通过在至少2代传代中评估生长和形态或原代人上皮细胞来测试培养基功能。 无细菌，真菌和支原体污染。
运输条件
介质冷冻运输
应用
仅供研究使用。 不用于治疗或诊断。
CnT-Prime, Epithelial Culture Medium
CnT-Prime is a fully defined, low calcium (0.07 mM) medium formulation designed for optimal growth and longevity of primary epithelial cells (skin, cornea, oral, mammary). It is completely free of animal or human-derived components. CnT-Prime contains a full range of amino acids, minerals, vitamins and organic compounds, plus growth factors to deliver strong cell proliferation, and cofactors to improve growth factor binding to cell surface receptors. CnT-Prime also contains PCT factors that maximise retention of proliferative progenitor cells, increase longevity, and minimise loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics.
SPECIES
Developed for primary human epithelial cells. May be used for other species as well, including mouse.
TISSUE TYPE
Epithelia
PACK SIZE
500 mL bottle, fully supplemented with growth factors. No further additions required.
PRODUCT USE
Developed specifically to deliver strong proliferation and extended longevity of primary epithelial cells in a fully defined environment. The inclusion of PCT factors increases retention of proliferative progenitor cells, and minimise loss through differentiation. Not recommended for use when inducing differentiation. For differentiation work, please use CnT-PR-D medium. When using CnT-PR for isolation, addition of the IsoBoost supplement (#CnT-ISO-50) has been found to double cell yields.
MEDIA TYPE
2D-Prolif
DEFINED
Yes
ACF
Yes
CULTURE CONDITION
This medium is designed for use in a 5% CO2 atmosphere. For suggested isolation, passaging, differentiation and freezing protocols, please visit the resources section of
NOTE
For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics. During isolation, antibiotics / antimycotics are recommended up until the end of P1.
STORAGE / SHELF LIFE
Store frozen below -15oC. For best before date, see label. To prepare medium for use, it is preferable to thaw overnight at 4oC. Alternatively, medium may be thawed at room temperature for approx 5-6 hours. If thawing at room temperature, ensure contents do not rise above 15oC at the end of the thawing process. Do not thaw in a hot water bath. Once thawed, medium has a remaining shelf-life of 6-weeks when stored at 4oC in the dark. Certain culture media components are very sensitive to light. Minimize light exposure at all times.
QUALITY CONTROL
Media composition is tested via osmolality, pH and the concentration of various ions. Media functionality is tested by evaluating growth and morphology or primary human epithelial cells over at least 2 passages. Free of bacteria, fungi and mycoplasma contamination.
SHIPPING CONDITION
Medium is shipped frozen.
INTENDED USE
For research use only. Not for use in therapy or diagnostics.