Dog BALP(Bone-specific alkaline phosphatase) ELISA Kit orb1146803
https://www.biorbyt.com/dog-balpbone-specific-alkalin-orb1146803.html
| Catalog Number | orb1146803 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone-specific alkaline phosphatase(BALP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bone-specific alkaline phosphatase(BALP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone-specific alkaline phosphatase(BALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone-specific alkaline phosphatase(BALP) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Canine |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.116 ng/mL |
| Protein Length | 3.5h |
| Alternative names | BALP, BSAP, HOPS, AP-TNAP, TNSALP, Bone Alkaline Phosphatase, Alkaline Phosphatase,Tissue-Nonspecific Isozyme Read less |
| Note | For research use only |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |