Human WWOX(WW domain-containing oxidoreductase) ELISA kit orb1146837
https://www.biorbyt.com/human-wwoxww-domain-containin-orb1146837.html
Catalog Number | orb1146837 |
---|---|
Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WW domain-containing oxidoreductase(WWOX). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to WW domain-containing oxidoreductase(WWOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WW domain-containing oxidoreductase(WWOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WW domain-containing oxidoreductase(WWOX) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Human |
Range | 0.32-20 ng/mL |
UniProt ID | Q9NZC7 |
Sensitivity | 0.115 ng/mL |
Protein Length | 3.5h |
Alternative names | WWOX, FOR, WOX1, WW domain-containing oxidoreductase, Fragile site FRA16D oxidoreductase Read less |
Note | For research use only |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |