Human FTO(Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit orb1146851
https://www.biorbyt.com/human-ftoalpha-ketoglutarate-orb1146851.html
| Catalog Number | orb1146851 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 0.16-10 ng/mL |
| UniProt ID | Q9C0B1 |
| Sensitivity | 0.059 ng/mL |
| Protein Length | 3.5h |
| Alternative names | FTO, KIAA1752, fat mass and obesity associated Read less |
| Note | For research use only |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
