Mouse Cort(Corticosterone) ELISA Kit orb1146853
https://www.biorbyt.com/mouse-cortcorticosterone-eli-orb1146853.html
Catalog Number | orb1146853 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Corticosterone(Cort) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticosterone(Cort). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticosterone(Cort) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Mouse |
Range | 1.57-100 ng/mL |
Sensitivity | 0.51 ng/mL |
Protein Length | 2h |
Alternative names | Corticosrone Read less |
Note | For research use only |
Sample Types | serum, plasma, urine and other biological fluids |
Expiration Date | Please enquire. |