Human SGPL1(Sphingosine 1 Phosphate Lyase 1) ELISA Kit orb1146855
https://www.biorbyt.com/human-sgpl1sphingosine-1-phos-orb1146855.html
| Catalog Number | orb1146855 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Lyase 1(SGPL1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Lyase 1(SGPL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Lyase 1(SGPL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Lyase 1(SGPL1) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 0.16-10 ng/mL |
| UniProt ID | O95470 |
| Sensitivity | 0.055 ng/mL |
| Protein Length | 3.5h |
| Alternative names | SPL, S1PL, Sphingosine-1-phosphate aldolase Read less |
| Note | For research use only |
| Sample Types | Tissue homogenates and other biological fluids. |
| Expiration Date | Please enquire. |
