Horse TNNI3(Troponin I Type 3, Cardiac) ELISA Kit orb1146876 https://www.biorbyt.com/horse-tnni3troponin-i-type-3-orb1146876.html
| Catalog Number | orb1146876 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 3, Cardiac(TNNI3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Troponin I Type 3, Cardiac(TNNI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 3, Cardiac(TNNI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 3, Cardiac(TNNI3) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Equine |
| Range | 31.25-2000 pg/mL |
| UniProt ID | Q5PYI0 |
| Sensitivity | 11.9 pg/mL |
| Protein Length | 3.5h |
| Alternative names | cTnI, TNNC1, CMH7, C-TnI, Troponin I Type 3, Cardiac troponin I, Troponin I Read less |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
