Horse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit orb1146877 https://www.biorbyt.com/horse-ctnttnnt2troponin-t-ty-orb1146877.html
Catalog Number | orb1146877 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 2, Cardiac(cTnT/TNNT2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Troponin T Type 2, Cardiac(cTnT/TNNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 2, Cardiac(cTnT/TNNT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin T Type 2, Cardiac(cTnT/TNNT2) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Equine |
Range | 15.63-1000 pg/mL |
Sensitivity | 6 pg/mL |
Protein Length | 3.5h |
Alternative names | CMH2, cTnT, TnTc, Cardiomyopathy,Hypertrophic 2, Cardiac muscle troponin T, TNNT2, Troponin T Type 2, Cardiac Read less |
Note | For research use only |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |