10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit orb1146884 https://www.biorbyt.com/10-hete10-hydroxyeicosatetrae-orb1146884.html
| Catalog Number | orb1146884 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 10-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 10-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 10-HETE in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | All |
| Range | 12.5-800 ng/mL |
| Sensitivity | 3.95 ng/mL |
| Protein Length | 2h |
| Alternative names | 10-HETE Read less |
| Note | For research use only |
| Sample Types | Serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
