Pig BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit orb1146907 https://www.biorbyt.com/pig-bmgb2-mgbeta-2-microglob-orb1146907.html
Catalog Number | orb1146907 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin(BMG/b2-MG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-2-Microglobulin(BMG/b2-MG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin(BMG/b2-MG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-2-Microglobulin(BMG/b2-MG) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Porcine |
Range | 3.13-200 ng/mL |
UniProt ID | Q07717 |
Sensitivity | 1.19 ng/mL |
Protein Length | 3.5h |
Alternative names | Beta 2M, BMG, B2MG, b2M, Beta-2-Microglobulin Read less |
Note | For research use only |
Sample Types | Serum, plasma, urine and other biological fluids |
Expiration Date | Please enquire. |