Zebrafish 5-HT(5-Hydroxytryptamine) ELISA Kit orb1146916 https://www.biorbyt.com/zebrafish-5-ht5-hydroxytrypta-orb1146916.html
| Catalog Number | orb1146916 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Zebrafish |
| Range | 14.07-900 ng/mL |
| Sensitivity | 3.97 ng/mL |
| Protein Length | 2h |
| Alternative names | 5HT; Serotonin; N-Methyl-Gamma Read less |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates |
| Expiration Date | Please enquire. |
