Hamster IL4(Interleukin 4) ELISA Kit orb1146927 https://www.biorbyt.com/hamster-il4interleukin-4-eli-orb1146927.html
| Catalog Number | orb1146927 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4(IL4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 4(IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4(IL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 4(IL4) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Hamster |
| Range | 15.63-1000 pg/mL |
| UniProt ID | Q60440 |
| Sensitivity | 5.7 pg/mL |
| Protein Length | 3.5h |
| Alternative names | IL-4, BSF1, BCGF1 , B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra Read less |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
