Rat PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit orb1146935
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Catalog Number | orb1146935 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat F protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Rat |
Range | 15.6-1000 ng/mL |
Sensitivity | 6.2 ng/mL |
Protein Length | 2h |
Alternative names | PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1 Read less |
Note | For research use only |
Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
Expiration Date | Please enquire. |