Rat GSTs(Glutathione S Transferases) ELISA Kit orb1146940 https://www.biorbyt.com/rat-gstsglutathione-s-transfe-orb1146940.html
| Catalog Number | orb1146940 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferases(GSTs). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione S Transferases(GSTs). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferases(GSTs), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferases(GSTs) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Rat |
| Range | 0.16-10 ng/mL |
| UniProt ID | O35543 |
| Sensitivity | 0.056 ng/mL |
| Protein Length | 3.5h |
| Alternative names | GSTs, GST-ST, GST ST Read more... |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
