Human IFNA17(Interferon alpha-17) ELISA Kit orb1146951
https://www.biorbyt.com/human-ifna17interferon-alpha-orb1146951.html
| Catalog Number | orb1146951 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon alpha-17(IFNA17). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon alpha-17(IFNA17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon alpha-17(IFNA17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon alpha-17(IFNA17) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 15.63-1000 pg/mL |
| UniProt ID | P01571 |
| Sensitivity | 5.7 pg/mL |
| Protein Length | 3.5h |
| Alternative names | Interferon alpha-88, Interferon alpha-T, IFN-alpha-17, Interferon alpha-17 Read less |
| Note | For research use only |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
