Rabbit GAPDH ELISA Kit orb1173551
https://www.biorbyt.com/rabbit-gapdh-elisa-kit-orb1173551.html
| Catalog Number | orb1173551 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Tested applications | ELISA |
| Reactivity | Rabbit |
| Range | 1.57-100 ng/mL |
| UniProt ID | P46406 |
| Sensitivity | 0.56 ng/mL |
| Protein Length | 3.5h |
| Alternative names | G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH Read more... |
| Note | For research use only |
| Sample Types | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
