Sheep SOD ELISA Kit orb1173555
https://www.biorbyt.com/sheep-sod-elisa-kit-orb1173555.html
Catalog Number | orb1173555 |
---|---|
Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep SOD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep SOD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep SOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep SOD in the samples is then determined by comparing the OD of the samples to the standard curve. |
Tested applications | ELISA |
Reactivity | Sheep |
Range | 0.16-10 U/mL |
UniProt ID | P09670 |
Sensitivity | 0.066 U/mL |
Protein Length | 3.5h |
Alternative names | Superoxide Dismutases Read more... |
Note | For research use only |
Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
Expiration Date | Please enquire. |