Sheep PON1 ELISA Kit orb1173557
https://www.biorbyt.com/sheep-pon1-elisa-kit-orb1173557.html
| Catalog Number | orb1173557 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paraoxonase 1(PON1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Paraoxonase 1(PON1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paraoxonase 1(PON1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paraoxonase 1(PON1) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Tested applications | ELISA |
| Reactivity | Sheep |
| Range | 1.25-80 ng/mL |
| Sensitivity | 0.51 ng/mL |
| Protein Length | 3.5h |
| Alternative names | ESA, PON, Esterase A, Serum paraoxonase/arylestera Read more... |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
