Rabbit F1+2 ELISA Kit orb1173568
https://www.biorbyt.com/rabbit-f12-elisa-kit-orb1173568.html
| Catalog Number | orb1173568 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prothrombin Fragment 1+2(F1+2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prothrombin Fragment 1+2(F1+2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prothrombin Fragment 1+2(F1+2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prothrombin Fragment 1+2(F1+2) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Tested applications | ELISA |
| Reactivity | Rabbit |
| Range | 3.13-200 ng/mL |
| Sensitivity | 1.35 ng/mL |
| Protein Length | 3.5h |
| Alternative names | F1+2 Read more... |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
