Rabbit sC5b-9 ELISA Kit orb1173569 https://www.biorbyt.com/rabbit-sc5b-9-elisa-kit-orb1173569.html
| Catalog Number | orb1173569 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Terminal Complement Complex C5b-9(sC5b-9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Terminal Complement Complex C5b-9(sC5b-9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Terminal Complement Complex C5b-9(sC5b-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Terminal Complement Complex C5b-9(sC5b-9) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Tested applications | ELISA |
| Reactivity | Rabbit |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.10 ng/mL |
| Protein Length | 3.5h |
| Alternative names | MAC, Membrane Attack Complex, C5b9, TCC C5b-9 Read more... |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
