Rat MBL1 ELISA Kit orb1173622
https://www.biorbyt.com/rat-mbl1-elisa-kit-orb1173622.html
| Catalog Number | orb1173622 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose-binding protein A(MBL1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mannose-binding protein A(MBL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose-binding protein A(MBL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose-binding protein A(MBL1) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Tested applications | ELISA |
| Reactivity | Rat |
| Range | 0.16-10 ng/mL |
| UniProt ID | P19999 |
| Solubility (25°C) | 10 ng/mL |
| Sensitivity | 0.059 ng/mL |
| Alternative names | MBL1,Mbl1Mannose-binding protein A, MBP-A, Mannan- Read more... |
| Note | For research use only |
| Application notes | Sandwich |
| Sample Types | Serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
