Human fT4(Free Thyroxine) ELISA Kit orb1736533
https://www.biorbyt.com/human-ft4free-thyroxine-elis-orb1736533.html
| Catalog Number | orb1736533 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human fT4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human fT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human fT4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 1.25-80 pg/mL |
| Sensitivity | 0.48 pg/mL |
| Alternative names | FTI Read more... |
| Note | For research use only |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
