Human Acetyl-H3(Acetyl Histone H3) ELISA Kit orb1736534
https://www.biorbyt.com/human-acetyl-h3acetyl-histone-orb1736534.html
| Catalog Number | orb1736534 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Acetyl-H3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Acetyl-H3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Acetyl-H3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Acetyl-H3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 0.32-20 ng/mL |
| UniProt ID | P68431 |
| Sensitivity | 0.16 ng/mL |
| Alternative names | H3 histone family member E pseudogene; H3 histone Read more... |
| Note | For research use only |
| Sample Types | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
