Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit orb1736561
https://www.biorbyt.com/human-23-dpg23-diphosphogly-orb1736561.html
| Catalog Number | orb1736561 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 0.32-20 μmol/mL |
| Sensitivity | 0.14 μmol/mL |
| Alternative names | 2,3DPG Read more... |
| Note | For research use only |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| Expiration Date | Please enquire. |
