Human Cot(Cotinine) ELISA Kit orb1736620
https://www.biorbyt.com/human-cotcotinine-elisa-kit-orb1736620.html
Catalog Number | orb1736620 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Cot protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Cot. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cot in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Human |
Range | 1.57-100 ng/mL |
Sensitivity | 0.61 ng/mL |
Alternative names | Cotinine Read more... |
Note | For research use only |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |