Mouse Anti-IAA(Anti-Insulin Autoantibody) ELISA Kit orb1736667
https://www.biorbyt.com/mouse-anti-iaaanti-insulin-au-orb1736667.html
| Catalog Number | orb1736667 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-IAA in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Mouse |
| Range | 0.78-50 ng/mL |
| Sensitivity | 0.31 ng/mL |
| Alternative names | Anti-Insulin; Antiinsulin; Insulin Antibodies Read more... |
| Note | For research use only |
| Sample Types | Serum, plasma and other biological fluids. |
| Expiration Date | Please enquire. |