Simian C5b-9(Terminal Complement Complex C5b-9) ELISA Kit orb1736768
https://www.biorbyt.com/simian-c5b-9terminal-compleme-orb1736768.html
| Catalog Number | orb1736768 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian C5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian C5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian C5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian C5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Primate |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.14 ng/mL |
| Alternative names | MAC; Membrane Attack Complex; C5b-9; Terminal Comp Read more... |
| Note | For research use only |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
