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Porcine KRT18 ELISA Kit orb781895
https://www.biorbyt.com/porcine-krt18-elisa-kit-orb781895.html
| Catalog Number | orb781895 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18(KRT18). |
| Tested applications | ELISA |
| Reactivity | Porcine |
| Range | 0.16-10 ng/mL |
| Concentration | 10 ng/mL |
| Sensitivity | 0.054 ng/mL |
| Protein Length | 3.5h |
| Alternative names | CK18 ELISA Kit, CYK18 ELISA Kit, K18 ELISA Kit, Cy Read more... |
| Note | For research use only |
| Application notes | Standard: 10 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig KRT18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig KRT18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig KRT18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig KRT18 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
| Expiration Date | Please enquire. |