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Human Anti-AsAb ELISA Kit orb781908
https://www.biorbyt.com/human-anti-asab-elisa-kit-orb781908.html
| Catalog Number | orb781908 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Anti-Sperm Antibody Antibody(Anti-AsAb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Anti-Sperm Antibody Antibody(Anti-AsAb). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 3.13-200 ng/mL |
| Concentration | 200 ng/mL |
| Sensitivity | 1.17 ng/mL |
| Protein Length | 2h, 30min |
| Alternative names | Anti-Spermatozoa Antibodies ELISA Kit, Sperm Antib Read more... |
| Note | For research use only |
| Application notes | Standard: 200 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-AsAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-AsAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |