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Porcine IGFBP7 ELISA Kit orb781914
https://www.biorbyt.com/porcine-igfbp7-elisa-kit-orb781914.html
| Catalog Number | orb781914 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 7(IGFBP7). |
| Tested applications | ELISA |
| Reactivity | Porcine |
| Range | 0.32-20 ng/mL |
| Concentration | 100 ng/mL |
| Sensitivity | 0.135 ng/mL |
| Protein Length | 3.5h |
| Alternative names | FSTL2 ELISA Kit, IGFBP-7v ELISA Kit, MAC25 ELISA K Read more... |
| Note | For research use only |
| Application notes | Standard: 20 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IGFBP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IGFBP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IGFBP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma and other biological fluids. |
| Expiration Date | Please enquire. |