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Other cAMP ELISA Kit orb781952
https://www.biorbyt.com/other-camp-elisa-kit-orb781952.html
| Catalog Number | orb781952 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyclic Adenosine Monophosphate(cAMP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclic Adenosine Monophosphate(cAMP). |
| Tested applications | ELISA |
| Reactivity | All |
| Range | 312.5-20000 pg/mL |
| Concentration | 20000 pg/mL |
| Sensitivity | 92.5 pg/mL |
| Protein Length | 2h |
| Alternative names | c-AMP ELISA Kit, 3'-5'-Cyclic Adenosine Monophosph Read more... |
| Note | For research use only |
| Application notes | Standard: 20000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |