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Canine TNNT2 ELISA Kit orb781963
https://www.biorbyt.com/canine-tnnt2-elisa-kit-orb781963.html
| Catalog Number | orb781963 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 2, Cardiac(TNNT2). |
| Tested applications | ELISA |
| Reactivity | Canine |
| Range | 62.5-4000 pg/mL |
| Concentration | 4000 pg/mL |
| Sensitivity | 23.9 pg/mL |
| Protein Length | 3.5h |
| Alternative names | CMH2 ELISA Kit, cTnT ELISA Kit, TnTc ELISA Kit, Ca Read more... |
| Note | For research use only |
| Application notes | Standard: 4000 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
| Expiration Date | Please enquire. |