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Other IP3 ELISA Kit orb781984
https://www.biorbyt.com/other-ip3-elisa-kit-orb781984.html
| Catalog Number | orb781984 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Inositol Triphosphate(IP3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inositol Triphosphate(IP3). |
| Tested applications | ELISA |
| Reactivity | All |
| Range | 156.25-10000 pg/mL |
| Concentration | 10000 pg/mL |
| Sensitivity | 49.3 pg/mL |
| Protein Length | 2h |
| Alternative names | InsP3 ELISA Kit, Inositol 1,4,5-Trisphosphate ELIS Read more... |
| Note | For research use only |
| Application notes | Standard: 10000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |