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Mouse IL-27(Interleukin 27) ELISA Kit orb1146720
https://www.biorbyt.com/mouse-il-27interleukin-27-el-orb1146720.html
| Catalog Number | orb1146720 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 27(IL-27). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 27(IL-27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 27(IL-27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 27(IL-27) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Mouse |
| Range | 31.25-2000 pg/mL |
| Concentration | 2000 pg/mL |
| UniProt ID | Q8K3I6 |
| Sensitivity | 18.75 pg/mL |
| Protein Length | 3.5h |
| Alternative names | IL-27, IL-27A, IL27p28, IL30, MGC71873, p28, Read less |
| Note | For research use only |
| Application notes | Standard: 2000 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |