F-Testo(Free Testosterone) ELISA Kit orb1146748
https://www.biorbyt.com/f-testofree-testosterone-eli-orb1146748.html
Catalog Number | orb1146748 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FreeTestosterone(F-TESTO) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FreeTestosterone(F-TESTO). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FreeTestosterone(F-TESTO) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | All |
Range | 31.25-2000 pg/mL |
Concentration | 2000 pg/mL |
Sensitivity | 18.76 pg/mL |
Protein Length | 2h |
Alternative names | F-TESTO Read less |
Note | For research use only |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |