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4-HNE(4-Hydroxynonenal) ELISA Kit orb1146751
https://www.biorbyt.com/4-hne4-hydroxynonenal-elisa-orb1146751.html
| Catalog Number | orb1146751 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | All |
| Range | 0.63-40 ng/mL |
| Concentration | 40 ng/mL |
| Sensitivity | 0.38 ng/mL |
| Protein Length | 2h |
| Alternative names | 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal Read more... |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |