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Rat BAK1(Bcl2 Antagonist/Killer 1) ELISA Kit orb1146762
https://www.biorbyt.com/rat-bak1bcl2-antagonistkille-orb1146762.html
| Catalog Number | orb1146762 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Antagonist/Killer 1(BAK1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Antagonist/Killer 1(BAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Antagonist/Killer 1(BAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Antagonist/Killer 1(BAK1) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Rat |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.054 ng/mL |
| Protein Length | 3.5h |
| Alternative names | BAK, BAK-LIKE, BCL2L7, CDN1, Bcl-2 Homologous Antagonist/Killer, Apoptosis regulator BAK, Bcl-2-like protein 7 Read less |
| Note | For research use only |
| Sample Types | Tissue homogenates, cell lysates and other biological fluids |
| Expiration Date | Please enquire. |