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Human MKRN3(makorin ring-finger protein 3) ELISA Kit orb1146804
https://www.biorbyt.com/human-mkrn3makorin-ring-finge-orb1146804.html
| Catalog Number | orb1146804 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to makorin ring-finger protein 3(MKRN3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to makorin ring-finger protein 3(MKRN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain makorin ring-finger protein 3(MKRN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of makorin ring-finger protein 3(MKRN3) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 15.63-1000 pg/mL |
| UniProt ID | Q13064 |
| Sensitivity | 6.5 pg/mL |
| Protein Length | 3.5h |
| Alternative names | Probable E3 ubiquitin-protein ligase makorin-3, RING finger protein 63, Zinc finger protein 127, D15S9, RNF63, ZNF127 Read less |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |