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Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit orb1146806
https://www.biorbyt.com/mouse-bcat1branched-chain-ami-orb1146806.html
| Catalog Number | orb1146806 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Mouse |
| Range | 15.63-1000 pg/mL |
| UniProt ID | P24288 |
| Sensitivity | 6.5 pg/mL |
| Protein Length | 3.5h |
| Alternative names | Branched-chain-amino-acid aminotransferase, cytosolic, BCAT (c) , Protein ECA39, Eca39, Bcat1 Read less |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |